Assistant Biology Univ. College Professor Resume-2

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Contact Information:

Objective:

To use my knowledge in best possible and innovative way for the betterment of the environment and to secure a position in Biotech environment

Resume Summery:

• Solid analytic and technical skills in developmental biology, molecular biology, protein chemistry, genetics, and in vivo animal studies
• Selfmotivated, hard working, quick learner and a good team player

Education:

• Ph.D. in Cell and Developmental Biology at the Department of Anatomy and Cell Biology, College of Medicine, University of Florida, December 1998
• Majoring Medicine at the Department of Medicine, Second Military Medical, University, Shanghai, P.R. of China. July 1986

Membership and Honor:

• Member of American Society for Cell Biology since 1995
• 2000 National Research Service Award (1 F32 HL1038101)
• Associate Member of American Society for Hematology since 2002
• Minisymposium speaker at American Society for Hematology, 2003
• Travel Award recipient of American Society for Hematology, 2003

Professional Experience:

Research Assistant Professor at Dept. of Pharmacology, UNC at Chapel

Hill. 2005 Present

• 

  Research Assistant
  My current research focuses on the fundamental role of CIB1 in vivo.
• Using mouse genetic model as well as cell and molecular biology and functional genomics, generated a mouse model lacking CIB1. Found the CIB1 is essential for spermatogenesis. It also plays roles in angiogenesis, cell cycle regulation and hemostasis in mouse.

Postdoctoral Fellow at Department of Pharmacology, UNC at Chapel Hill. 1999 2004: Advisor: Dr. Leslie V. Parise

• My research in Dr. Parise’s lab focuses on the function and signaling properties of a regulatory protein called CIB1.
• Used megakaryocytes derived from cultured mouse bone marrow stem cells to study integrin a IIbb°3 and CIB1 function and found CIB1 is an endogenous inhibitor of integrand.
• Generated a CIB1 knockout mouse and a CIB1 transgenic mice to study the role of CIB1 in mouse embryonic development and its role in integrin signaling pathway
• Applied and funded by NIH for the study of CIB1 function in integrin signaling pathway

Graduate research associate at Dept. Anatomy and Cell Biology, Univ. of Florida. 19931998: Advisor: Dr. William A. Dunn

• My Research focused on the regulatory mechanism of protein degradation pathway in yeast systems. In this position,
• Successfully established a study model for autophagy in yeast Pichia pastoris using genetic, molecular, cell biology and biochemistry approaches
• Identified 8 complementation groups of glucose induced selective microautophagy (gsa) mutants in yeast Pichia pastoris
• Cloned two gsa genes that regulate the microautophagy by genomic DNA library screening
• Among the first scientists to discover a second ubiquitinlike protein degradation pathway
• Both studies were published and the combined lab results were orally presented at a minisymposium in a 1999 ASCB meeting

Professional Skills:

Experiment design and analysis

• Biochemical analysis: Enzyme activity, protein chemistry including protein purification, Western blot, and immunoprecipitation, ELISA
• Molecular biology: DNA expression library screening, vector construction, site directed mutagenesis, bacterial and yeast transformation, gene screen and recovery from genomic library, PCR, Real timePCR, Northern, Southern, analysis of gene expression, gene targeting, gene knockout, Protein expression studies using retrovirus propagation and transfection of cell line and bone marrow cells.
• Cell biology: different mammalian cell lines culture, cell transfection and assays, flow cytometry analysis,immunoassay, light and electron microscopy,immunofluorescence as well as digital imaging analysis
• Animal studies: In vivo transgenic and knockout mice model studies, animal maintaining and animal surgery and tissue histology, bone marrow stem cell culture and studies
• Computer Skills: extensive bioinformatics skills using software and web resource such as computer analysis of gene sequence, genome mapping and mining, computer protein structure studies and quantification of protein, Photoshop and statistic analysis.

Recent Presentations:

• 

  Assistant Biology Professor
  W. Yuan, T.L. Leisner, A.W. McFadden, S. Clark, S. Hiller, N. Maeda, D.A. O’Brien and L.V. Parise. 2006. CIB1 is essential for mouse spermatogenesis. American Society of Cell Biology, San Diego, December 2006
• M.A. Zayed, A.W. McFadden, W. Yuan, M.E. Hartnett, J. Faber & L.V. Parise. 2006. Essential Role of CIB1 in Endothelial Cell Function and In Vivo IschemiaInduced Angiogenesis. American Society of Hematology. Orlando, December 2006
• J. DeNofiro, W. Yuan, M Rojas & L.V. Parise. 2006. Elevations in adhesive plasma proteins in the CIB1 knockout mouse. Midwest Platelet Conference. Chicago, October 2006
• L.V. Parise, W. Yuan, A.W. McFadden, Z.Wang, M.K. Larson, T.M. Leisner, J. DeNofrio, J. Liu, S. Clark. 2005. Genetic Manipulation of Megakaryocytes to Study Platelet Integrin Regulation (oral presentation at 2005 ISTH meeting, Sydney, Australia)
• W. Yuan, A.W. McFadden, Z. Wang, M.K. Larson, C. BoudignonProudhan, L.V. Parise. 2003. CIB1 is an Endogenous Inhibitor of InsideOut Integrin *IIb*3 Activation in Primary Murine Megakaryocytes. Blood 102:160a, #555, 2003 (oral presentation at 2003 ASH meeting, San Diego)

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